Our UHN programs and services are among the most advanced in the world. We have grouped our physicians, staff, services and resources into 10 medical programs to meet the needs of our patients and help us make the most of our resources.
University Health Network is a health care and medical research organization in Toronto, Ontario, Canada. The scope of research and complexity of cases at UHN has made us a national and international source for discovery, education and patient care.
Our 10 medical programs are spread across eight hospital sites – Princess Margaret, Toronto General, Toronto Rehab’s five sites, Toronto Western – as well as our education programs through the Michener Institute of Education at UHN. Learn more about the services, programs and amenities offered at each location.
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Find out how to get to and around our nine locations — floor plans, parking, public transit, accessibility services, and shuttle information.
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In order to view your tissue sections under a microscope, they must be stained to make the cells visible. Most staining techniques use dyes that selectively stain tissues and cells in many different ways. We do this to help the pathologist identify the type of tissue present, and visualize changes in normal pathology or the presence of non-normal elements such as bacteria or fungus.
One of the most common routine stains is Hematoxylin and Eosin, often referred to as an H&E. The combination of a basic dye and an acidic dye allows the different elements of the tissue to be demonstrated with different colors. For example, nuclei stain blue and cytoplasm, muscle and red blood cells are a range of bright pink to red.
Automated instruments in our laboratory allow large volumes of slides to be stained without significant hands-on time for technical staff. The run time is from 20 – 40 minutes per slide batch. The stained slides are now ready for coverslipping.
Racks of slides are moved from the stainer to the holding trough of a robotic coverslipper.
The purpose of the coverslipper is to apply a glass coverslip that completely covers the tissue section on the slide so that it's preserved. A drop of mounting media is dispensed onto the slide and the coverslip is placed on top. The mounting media acts as a glue to adhere the coverslip to the slide, and has an optical density close to that of glass so there is no distortion when viewing sections microscopically.
Quality control checks are performed before sending the slides to the pathologist. The slides prepared are documented in the LIS, and then delivered to the pathologist.